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目的:研究血管紧张素Ⅱ1型受体拮抗剂科素亚对长期大强度运动所致心脏纤维化的预防作用。方法:48只雄性Wistar大鼠随机分为4组:对照组,科素亚处理的对照组,运动组,运动+科素亚处理组。运动组大鼠给予16周大强度运动,科素亚处理的大鼠每天训练前口服科素亚每次(50毫克/公斤/天)。通过计算心系数和观察心脏的组织形态学评估和比较各组大鼠心脏肥大的程度,免疫印迹法检测和比较各组大鼠4个心腔内转化生长因子-β1、纤维连接蛋白1、基质金属蛋白酶-2、I型胶原蛋白和III型胶原蛋白的蛋白表达水平。结果:长期大强度运动可造成大鼠左心室心脏壁肥大和右心室胶原沉积,心房和右心室的主要纤维化生物标志物的蛋白表达水平显著增加。科素亚预处理能够减少主要纤维化生物标志物TGF-β1、FIBROECTIN-1、MMP-2、TIMP-1、COLLAGEN-I、COLLAGEN-III表达水平,但不能完全改变心脏重量与体重比值增大的状态。结论:科素亚可部分预防耐力运动训练所致的心脏纤维化,但不能彻底改善心脏肥大。
Objective: To investigate the preventive effect of angiotensin Ⅱ type 1 receptor antagonist Cozaar on cardiac fibrosis induced by long-term intensive exercise. Methods: Forty-eight male Wistar rats were randomly divided into 4 groups: control group, Cauxa control group, exercise group, exercise + cotyledone group. Rats in the exercise group were given intensive exercise for 16 weeks, and Cauxa-treated rats were orally administered with Cozaar (50 mg / kg / day) daily before training. By calculating the cardiac coefficient and observing the histopathology of the heart, the levels of cardiac hypertrophy in each group were evaluated and compared. The expression of transforming growth factor-β1, fibronectin 1, matrix Protein protease-2, type I collagen and type III collagen protein expression levels. RESULTS: Long-term high-intensity exercise induced left ventricular hypertrophy and right ventricular collagen deposition in rats. The protein expression of major fibrotic biomarkers in atria and right ventricle increased significantly. Cozaar preconditioning reduced the expression of TGF-β1, FIBROECTIN-1, MMP-2, TIMP-1, COLLAGEN-I and COLLAGEN-III as major fibrosis biomarkers but did not completely alter the ratio of heart weight to body weight status. CONCLUSION: Cozaar can partially prevent cardiac fibrosis induced by endurance exercise training, but it can not completely improve cardiac hypertrophy.