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目的探讨解偶联蛋白2(uncoupling protein 2,UCP2)在老年大鼠肝脏组织中的表达及其与衰老的关系。方法健康雄性SD大鼠30只,按年龄分为新生组、成年组和老年组,实验室适应性喂养7 d后断头处死,分别取各组大鼠肝组织测定丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)、三磷酸腺苷(adenosine triphosphate,ATP)的含量;通过免疫组织化学观察肝组织中UCP2蛋白的表达与分布,Western blot方法检测肝细胞线粒体内UCP2蛋白的表达并进行定量分析。结果老年组大鼠肝组织中MDA含量高于幼年组和成年组(P<0.01);老年组大鼠肝组织GSH含量显著低于幼年组和成年组(P<0.01);老年组的ATP水平比幼年组和成年组水平显著降低(P<0.01);老年组UCP2表达高于幼年组和成年组(P<0.01)。结论老年大鼠肝MDA含量升高、GSH含量下降,提示老年大鼠肝内活性氧(re-active oxygen species,ROS)水平较高,并引起UCP2表达增多,UCP2可能对延缓肝细胞的衰老起重要作用;UCP2的高表达可能是老年大鼠肝组织ATP下降的原因之一;肝组织UCP2表达水平主要受ROS水平的调节,但ATP水平不仅受到UCP2的调节,还受细胞的代谢状态的影响。
Objective To investigate the expression of uncoupling protein 2 (UCP2) in the liver of aged rats and its relationship with aging. Methods Thirty healthy male Sprague-Dawley rats were divided into three groups according to their age: neonatal group, adult group and old group. Rats in experimental group were sacrificed 7 d after adaptive diet. The malondialdehyde (MDA) ), Glutathione (GSH) and adenosine triphosphate (ATP). The expression and distribution of UCP2 protein in liver tissue were observed by immunohistochemistry. The expression of UCP2 protein in liver mitochondria was detected by Western blot Quantitative analysis. Results The content of MDA in the liver of the aged group was higher than that of the young group and the adult group (P <0.01). The content of GSH in the liver of the aged group was significantly lower than that in the young group and the adult group (P <0.01) (P <0.01). The expression of UCP2 in elderly group was higher than that in young group and adult group (P <0.01). Conclusion The content of MDA in the liver and the content of GSH in the senile rats are decreased, suggesting that the level of reactive oxygen species (ROS) in the liver is higher and the expression of UCP2 is increased in aged rats. UCP2 may play an important role in delaying the aging of hepatocytes UCP2 may be one of the reasons for the decrease of ATP in the liver of aged rats. The expression level of UCP2 in liver tissue is mainly regulated by the level of ROS, but the level of ATP is not only regulated by UCP2, but also influenced by the metabolic state of the cells .