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以野菊花为材料,顺次用石油醚、氯仿、乙酸乙酯、正丁醇、水抽提,抽提物终浓度相当于1g草药/ml,对5μmol/L ADP诱导的血小板聚集分别抑制30.8±20.2%、49.3±29.4%、51.9±22.8%、43.5±18.0%、13.7±1.6%。野菊花乙酸乙酯抽提物抑制5μmol/L ADP诱导的血小板聚集IC_(50)=1mg/ml。乙酸乙酯抽提物用硅胶柱制备分离得C1、C2、C3、C4、C5、C6六种结晶,经TLC检识分别为一个斑点。定性分析知为基黄酮化合物。在0.5mg/ml浓度下,对2μmol/L ADP诱导的血小板聚集后三种物质分别抑制78.9%、85.3%、83.5%。
The chrysanthemum was extracted with petroleum ether, chloroform, ethyl acetate, n-butanol and water. The final concentration of extract was equivalent to 1 g herb/ml, which inhibited the platelet aggregation induced by 5 μmol/L ADP 30.8 respectively. ±20.2%, 49.3±29.4%, 51.9±22.8%, 43.5±18.0%, 13.7±1.6%. The chrysanthemum ethyl acetate extract inhibited platelet aggregation induced by 5 μmol/L ADP IC 50 in 1 mg/ml. Ethyl acetate extracts were prepared on silica gel columns to obtain six crystals of C1, C2, C3, C4, C5, and C6, which were identified by TLC as one spot. Qualitative analysis is known as flavonoid compounds. At a concentration of 0.5 mg/ml, 78.9%, 85.3%, and 83.5% of the three substances were inhibited by 2 μmol/L ADP-induced platelet aggregation, respectively.