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目的探讨雷帕霉素(RPM)对大鼠CD4+CD25+FoxP3+调节性T细胞的影响。方法大鼠20只随机均分为两组:实验组RPM2mg·kg-1.d-1灌胃2周;对照组用生理盐水替代。无菌件下下腔静脉采血,并分离脾脏及胸腺,制备单个核细胞悬液。采用流式细胞术检测大鼠外周血、脾脏及胸腺内CD4+CD25+T细胞的占单个核细胞的比例,实时定量-PCR检测脾脏细胞FoxP3 mRNA表达,ELISA检测外周血血清转化生长因子β(TGF-β)和白细胞介素10(IL-10)含量。结果实验组外周血、脾脏和胸腺中CD4+CD25+T细胞的比例明显高于对照组(P<0.05)。实验组大鼠脾脏细胞FoxP3 mRNA表达为对照组的4.1倍。实验组TGF-β和IL-10显著高于对照组(P<0.05)。结论 RPM能诱导大鼠体内CD4+CD25+FoxP3+调节性T细胞增殖,且增加体内免疫抑制性细胞因子TGF-β和IL-10的分泌。
Objective To investigate the effect of rapamycin on CD4 + CD25 + FoxP3 + regulatory T cells in rats. Methods Twenty rats were randomly divided into two groups: experimental group RPM2mg · kg-1.d-1 for 2 weeks; control group with saline instead. Aseptic blood samples were collected from the inferior vena cava and spleens and thymus were isolated to prepare mononuclear cell suspensions. The ratio of CD4 + CD25 + T cells to mononuclear cells in peripheral blood, spleen and thymus were measured by flow cytometry. Foxp3 mRNA expression in spleen cells was detected by real-time quantitative PCR. Serum levels of transforming growth factor-beta TGF-β) and interleukin-10 (IL-10) levels. Results The proportion of CD4 + CD25 + T cells in peripheral blood, spleen and thymus in experimental group was significantly higher than that in control group (P <0.05). The expression of FoxP3 mRNA in the spleen cells of the experimental group was 4.1 times that of the control group. The levels of TGF-β and IL-10 in the experimental group were significantly higher than those in the control group (P <0.05). Conclusion RPM can induce the proliferation of CD4 + CD25 + FoxP3 + regulatory T cells and increase the secretion of immunosuppressive cytokines TGF-β and IL-10 in vivo.