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对牛大力组织培养瓶内复壮瓶外生根快繁技术进行了研究,用改良MS+6-BA0.4mg/L+KT0.4mg/L+NAA0.3mg/L为继代培养基,25d繁殖系数达5.0～7.0,复叶数达2～3张,继代苗在改良MS+KT0.4mg/L+IBA0.3mg/L培养基上复壮后,基部浸蘸ABT生根粉500mg/L或IBA300mg/L溶液约1min,扦插在黄心土:河沙:泥炭土(10:1:1)混合基质中,40d出主根2条,生根率达75%～85%。
The experiment was conducted to study the rooting and multiplication technology of bovine rehmanniae in vitro. The improved multiplication coefficient of 25d by using MS + 6-BA0.4mg / L + KT0.4mg / L + NAA0.3mg / L as the subculture medium, Up to 5.0-7.0, and the number of compound leaves was 2-3.After subplant replanting ABT rooting powder 500mg / L or IBA 300mg / L on MS + KT 0.4mg / L + IBA 0.3mg / L medium, L solution for about 1min, cuttings were mixed in a mixture of yellow heart soil: river sand and peat soil (10: 1: 1), and two rootlets were found after 40 days. The rooting rate reached 75% ~ 85%.