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The antitumor mechanism of etoposide (VP-16) is investigated using pulse radiolysis technology. The oxidizing mechanism of VP-16 is studied by sodium persulfate, and the reaction rate constant is 4.04×109 L·mol-1·s-1. The electron-transfer between VP-16 and tyrosine is observed and the reaction rate constant is 1.1×108 L·mol-1·s-1.