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用昼夜变温(20℃/10℃)-低温(4℃)、暖温(20℃)-低温(4℃)和低温(10℃)-低温(4℃)层积两阶段处理方法,研究变温层积处理过程中东北红豆杉种子生理生化特性的变化规律。结果表明:昼夜变温层积6个月后种胚长度和宽度分别由层积前的1.52和0.47 mm,增加到1.95和0.61 mm;昼夜变温-低温层积后种子可溶性糖含量达到46.3 mg/g,显著高于层积前;粗脂肪含量比层积前减少了3.8%;脱氢酶(TTC)活性持续增加,层积10个月后达到层积前的2倍多;葡萄糖六磷酸脱氢酶(G-6-PDH)活性在昼夜变温层积阶段下降,低温层积阶段上升;三磷酸腺苷(ATP)水平在昼夜变温层积过程中变化不大,而低温层积后ATP水平显著增加,达到层积前的3.9倍。昼夜变温-低温层积的种子裂口率为11.4%,出苗率为14.7%,显著高于暖温和低温处理,说明昼夜变温-低温处理效果优于暖温-低温和低温-低温处理。
A two-stage stratification method of diurnal (20 ℃ / 10 ℃) -low temperature (4 ℃), warm (20 ℃) -low temperature (4 ℃) and low temperature (4 ℃) Variation of Physiological and Biochemical Characteristics of Taxus cuspidata Sieb. The results showed that the seed germination length and width increased from 1.52 and 0.47 mm before stratification to 1.95 and 0.61 mm respectively after 6 months of stratification. After diurnal temperature-low temperature stratification, the soluble sugar content reached 46.3 mg / g , Which was significantly higher than that before stratification; crude fat content decreased by 3.8% than that before stratification; the activity of dehydrogenase (TTC) continued to increase, reaching more than 2 folds before stratification 10 months after stratification; dehydrogenation of glucose hexaphosphate The activity of G-6-PDH decreased during the phase of temperature change and accumulated during cold storage phase. The level of adenosine triphosphate (ATP) did not change much during the day and night temperature change, while the level of ATP increased significantly 3.9 times before stratification. The diurnal temperature-low temperature laminar seed split rate was 11.4%, the seedling emergence rate was 14.7%, which was significantly higher than that of warm and low temperature treatment, indicating that day and night temperature-low temperature treatment was better than warm temperature-low temperature and low temperature-low temperature treatment.