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昆虫线虫尤其是昆虫病原线虫可以部分替代化学农药防治有害昆虫。本文通过大蜡螟诱捕法对云南省玉溪市8县1区的昆虫线虫资源进行了调査。从270份土壤样品中分别得到57个昆虫线虫样本,分属于4个属,包括小杆科Rhabditidae的3个属,双胃科Diplogasteridae的1个属。结果表明,线虫的分布与土壤类型和植被类型密切相关,砂壤土、壤土中线虫检出率分别为100.0%、19.9%,较粘土12.1%的检出率高。在未受干扰的地块(林地和未耕地)线虫检出率为20.4%,受人类干扰的地块(烟田和农田)线虫检出率为16.1%,说明砂壤土和壤土适合昆虫线虫种群的建立,且在人活动较少的地块中线虫分布较多。用本土线虫在实验室条件下利用大蜡螟和蛴螬(棕色鳃金龟幼虫)对分离到的线虫进行致病力测定,结果表明:YN43(Oscheius sp.2)是大蜡螟最敏感的昆虫线虫品系,校正死亡率为76%;YN93(Oscheius sp.1)对蛴螬致病力最强,校正死亡率为54.5%,在防治云南地下害虫蛴螬方面具有很大的潜力。
Insect nematodes, especially entomopathogenic nematodes, can partially replace chemical pesticides in the control of harmful insects. In this paper, wax insect trap method of Yuxi City, Yunnan Province, 8 counties 1 insect nematode resources were investigated. 57 insect nematode samples were obtained from 270 soil samples, belonging to 4 genera, including 3 genera of Rhabditidae and 1 genera of Dipogasteridae. The results showed that the distribution of nematodes was closely related to soil types and vegetation types. The detection rates of nematodes in sandy loam soil and loam soil were 100.0% and 19.9%, respectively, which were higher than those of 12.1% clay. The detection rate of nematodes was 20.4% in undisturbed plots (woodland and uncultivated land), and the detection rate of nematodes in the disturbed land (tobacco and farmland) was 16.1%, indicating that sandy loam soil and loamy soil were suitable for insect nematode population The establishment of nematodes in the plots with less human activity. The pathogenicity of the isolated nematodes was determined using native C. elegans under laboratory conditions and the results showed that YN43 (Oscheius sp. 2) was the most sensitive insects nematode Strain, the corrected mortality rate was 76%; YN93 (Oscheius sp.1) had the strongest virulence and the corrected mortality rate was 54.5%, which had great potential in the control of Yunnan pests.