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目的:优化宽叶羌活挥发油超临界CO2萃取工艺。方法:利用超临界CO2流体萃取技术(SFE-CO2),在固定的萃取时间2h和气体流量65kg·h-1下,研究萃取温度和萃取压力对宽叶羌活挥发油提取率的影响,并采用气相色谱-质谱联用技术(GC-MS)进行分析萃取物的化学成分,以建立宽叶羌活挥发油提取的最佳工艺条件。结果:宽叶羌活挥发油提取的最佳工艺条件为萃取温度50℃、萃取压力25MPa,萃取时间2h,气体流量65kg·h-1,挥发油提取率达8.80%,约为水蒸气蒸馏法(SD)的6倍。超临界萃取挥发油中共鉴定出159种化合物,其中含Cl、S、B、F、Si的化合物在相关研究中均未见报道。结论:温度和压力是影响超临界CO2萃取宽叶羌活挥发油提取效率和稳定性的关键。
Objective: To optimize the supercritical CO2 extraction of volatile oil from Rhizoma et Radix notopterygii. Methods: SFE-CO2 was used to study the effects of extraction temperature and extraction pressure on the extraction rate of Volatile Oil from Rhizoma et Radix Notopterygii, with fixed extraction time of 2h and gas flow rate of 65kg · h-1. Chromatography - Mass Spectrometry (GC-MS) analysis of the chemical composition of the extract to establish a broad-leaved volatile oil extraction of the best processing conditions. Results: The optimal extraction conditions were: extraction temperature 50 ℃, extraction pressure 25MPa, extraction time 2h, gas flow rate 65kg · h-1, volatile oil extraction rate of 8.80%, about the steam distillation (SD) 6 times. A total of 159 compounds were identified in the supercritical fluid extraction. Among these compounds, none of the compounds containing Cl, S, B, F and Si were reported. Conclusion: Temperature and pressure are the key factors affecting the extraction efficiency and stability of Volatile Oil from Sophorae Radix extract by supercritical CO2.