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提出一个综合聚丙酰胺凝胶电泳(SDS-PAGE)分离和2,3-二氨基萘(DAN)柱前衍生正相液体色谱测定硒的方法(DAN-HPLC-FLD),分离测定了高硒地区人血浆中的含硒蛋白.在所鉴定的5种含硒蛋白中,其中至少有3种是从未明确鉴定过的含硒蛋白.
A method for the determination of selenium by integrated polyacrylamide gel electrophoresis (SDS-PAGE) and normal-phase liquid chromatography with 2,3-diaminonaphthalene (DAN) pre-column derivatization (DAN-HPLC-FLD) Among the five selenoproteins identified, at least three of them are selenoproteins that have never been specifically identified.