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目的探讨加味大柴胡汤(MMDB)对重症胆管炎(ACST)大鼠TLR4表达的影响。方法大鼠分为正常组、造模组、减压组、中药组。制作ACST大鼠模型后,分别于24、48及72h3个时点检测内毒素、肝功能及用RT-PCR检测TLR4mRNA的表达。结果造模组内毒素水平和TLR4含量均比正常组高;MMDB配合胆管减压治疗后,内毒素水平下降,TLR4表达降低,总胆红素降低,肝脏损害减轻。结论MMDB可以通过下调TLR4mRNA表达来降低血浆内毒素水平,从而减轻肝脏损害。
Objective To investigate the effect of Modified DaChaiHu Decoction (MMDB) on the expression of TLR4 in rats with severe cholangitis (ACST). Methods Rats were divided into normal group, model group, decompression group, and traditional Chinese medicine group. After the ACST rat model was established, endotoxin and liver function were detected at 24, 48, and 72 hours, and TLR4 mRNA expression was detected by RT-PCR. Results The level of endotoxin and TLR4 were higher in the model group than in the normal group. After treatment with MMDB combined with decompression of the bile duct, endotoxin levels decreased, TLR4 expression decreased, total bilirubin decreased, and liver damage decreased. Conclusion MMDB can reduce plasma endotoxin levels by down-regulating TLR4 mRNA expression and thus reduce liver damage.