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目的了解无锡市诺如病毒流行特征及主要流行株的基因型。方法采集2013年无锡市哨点医院监测的409份急性腹泻者的粪便标本和9起诺如病毒暴发疫情的病例标本,采用实时荧光定量PCR检测诺如病毒GⅠ和GⅡ基因,对GⅡ基因阳性标本进一步进行扩增及测序分型。结果哨点监测病例诺如病毒阳性率为9.78%(40/409)。所测36株诺如病毒共有5种基因型,依次为GⅡ.Pe/GⅡ.4型28株(77.78%)、GⅡ.P12/GⅡ.3型4株(11.11%)、GⅡ.P16/GⅡ.13型2株(5.56%)、GⅡ.P7/GⅡ.6和GⅡ.16各1株(2.78%)。2013年暴发疫情病例所测35株诺如病毒共4种基因型,依次为28株GⅡ.Pe/GⅡ.4型(80.0%)、4株GⅡ.7(11.43%)、3株GⅡ.12/GⅡ.3(8.57%)。结论诺如病毒是无锡地区非细菌性腹泻最常见的病原体,无锡地区诺如病毒流行株具有多种基因型,存在不同基因型间重组株。GⅡ.Pe/GⅡ.4型(Sydney_2012)为2013年无锡地区绝对优势流行株。
Objective To understand the epidemiological characteristics of Norovirus in Wuxi and the genotypes of major epidemic strains. Methods Stool specimens from 409 acute diarrhea patients and 9 outbreaks of norovirus were collected from sentinel hospital in Wuxi City in 2013. Real-time PCR was used to detect the GⅠ and GⅡ genes of Norovirus and the positive samples of GⅡ gene Further amplification and sequencing typing. Results The positive rate of norovirus in sentinel surveillance was 9.78% (40/409). Totally 36 genotypes of norovirus were detected, including 5 genotypes of GⅡ.Pe / GⅡ.4 type (77.78%), GⅡ.P12 / GⅡ.3 type 4 (11.11%), GⅡ.P16 / GⅡ 2 (5.56%), 1 (2.78%) of GⅡ.P7 / GⅡ.6 and GⅡ.16. There were 4 genotypes of 35 Norovirus detected in outbreak cases in 2013, followed by 28 GⅡ.Pe / GⅡ.4 (80.0%), 4 GⅡ.7 (11.43%) and 3 GⅡ.12 /GII.3(8.57%). Conclusion Norovirus is the most common non-bacterial diarrhea pathogen in Wuxi. Norovirus epidemic strains in Wuxi have multiple genotypes, and recombinants exist between different genotypes. GⅡ.Pe / GⅡ.4 (Sydney_2012) was the absolutely dominant strain in Wuxi in 2013.