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目的利用口服供者脾细胞延长受者大鼠皮肤存活时间,检测调节性T细胞在移植前后的变化,探讨口服免疫耐受的机制。方法以纯系SD大鼠为供者,纯系Wistar大鼠为受者,行异体皮肤移植,将24只受者大鼠随机分为A组(对照组,口服PBS)、B组(每日口服SD大鼠脾细胞1×107个),C组(每日口服SD大鼠脾细胞5×107个),对受体进行450Rad60Co照射,然后检测受者外周血及脾脏CD4+CD25+、CD8+CD28-调节性T细胞(Treg),并对受者进行供者脾细胞导管灌胃,每次口服5×107个细胞,每日一次,7 d后检查迟发型超敏反应(DTH),并行皮肤移植,观察移植皮肤的存活时间,复查Treg两次。结果口服脾细胞后,DTH反应均出现明显的抗原特异性降低;C组的皮肤移植存活时间达到19.17 d±1.94 d,与前两组比较差异有显著性(P<0.01)。在口服供体脾细胞延长皮肤移植存活大鼠(C组)的外周血及脾脏中CD8+CD28-Treg均比口服前明显增高(P<0.01),并能维持2周以上。结论口服抗原可以引起受者对异基因抗原的特异性免疫反应降低,使受者的皮肤移植存活期延长,CD8+CD28-Treg在口服免疫耐受的机制中发挥了重要作用。
OBJECTIVE: To explore the mechanism of oral immune tolerance by using oral donor spleen cells to prolong the survival time of recipient rat skin and to detect the changes of regulatory T cells before and after transplantation. Methods Twenty-four recipients were randomly divided into group A (control group, oral PBS), group B (daily oral administration 1 × 107 spleen cells of SD rats), group C (5 × 107 spleen cells of oral SD rats), and the recipients were irradiated with 450Rad60Co. Then the levels of CD4 + CD25 +, CD8 + CD28 - Regulatory T cells (Tregs), and the donor spleen cells were intragastrically administered orally, each oral 5 × 107 cells once a day, 7 days after the detection of delayed type hypersensitivity (DTH), parallel skin Transplantation, observe the survival time of transplanted skin, review Treg twice. Results After oral administration of spleen cells, the DTH reaction showed obvious antigen-specific reduction. The skin graft survival time in group C reached 19.17 d ± 1.94 d, which was significantly different from the former two groups (P <0.01). CD8 + CD28-Treg in peripheral blood and spleen of oral donor spleen cells prolonged skin graft survival in group C was significantly higher than that before oral administration (P <0.01), and maintained for more than two weeks. Conclusion Oral antigens can cause the recipients to reduce the specific immune response to allogeneic antigens and prolong the survival of recipients. CD8 + CD28-Treg plays an important role in the mechanism of oral immune tolerance.