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目的 探讨188Re直接法标记生长抑素类似物RC 16 0的方法及观察其在小鼠体内的生物学分布。方法 酒石酸亚锡直接还原法进行RC 16 0的188Re标记 ,标记完成后加入抗坏血酸以防标记产物的再氧化。硅胶纸层析测定放化纯 ,并行小鼠体内分布实验。结果 188Re RC 16 0放化纯为 96 .2 % ,游离188Re为 0 .8% ,放射性胶体为 3.0 %。加抗坏血酸组在标记后 2 4h放化纯为 85 % ,对照组为 5 0 %。注射后 0 .5～ 1.0h血液中放射性下降了 87.2 % ,肾脏无明显浓集 ,标记物 2 4h内由消化系统排出体外。结论 188Re直接法标记RC 16 0放化纯高 ,方法简便 ,抗坏血酸的加入增加了标记物的稳定性。188Re RC 16 0在小鼠体内血液清除较快 ,经消化系统排除。
Objective To investigate the method of direct labeling of somatostatin analogue RC16 0 by 188Re and to observe its biological distribution in mice. Methods The 188Re labeling of RC160 was performed by the direct stannous tartrate reduction method. Ascorbic acid was added to prevent the reoxidation of the labeled product. Silica gel chromatography determination of radioactive pure, parallel mice in vivo distribution experiment. Results 188Re RC 16 0 was 96.2% radiochemically pure, free 188Re was 0.8% and radioactive colloid was 3.0%. The ascorbate group was 85% pure at 24 hours after labeling, while the control group was 50%. The radioactivity in blood decreased by 87.2% at 0.5-1.0 hours after injection, with no significant concentration in the kidney. The markers were excreted by the digestive system within 24 hours. Conclusion 188Re direct labeled RC16 0 pure high purity, the method is simple, the addition of ascorbic acid increased the stability of the marker. 188Re RC 16 0 Rapid blood clearance in mice and elimination by the digestive system.