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目的:研究鞣花酸对高脂饮食引起的大鼠肥胖预防的影响。方法:将40只SD雄性大鼠按体重随机分为鞣花酸高剂量组(高脂饮食+30 mg/kg/d鞣花酸,EAH),鞣花酸低剂量组(高脂饮食+10 mg/kg/d鞣花酸,EAL),阳性对照组(高脂饮食+37.8mg/kg/d奥利司他,O),模型对照组(高脂饮食+10 mL/kg/d 0.5%CMC钠溶液,HFD)和空白对照组(普通饮食+10 mL/kg/d 0.5%CMC钠溶液,ND),连续给药10周,测量体重、进食量、体脂、肝重、肾重,计算脏器系数和脂肪系数,测定血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C);HE染色观察脂肪细胞和肝脏组织细胞形态。结果:干预10周后,与模型对照组比较,鞣花酸高、低剂量组大鼠体重和进食量均显著下降(P<0.05),脂肪系数显著降低(P<0.001);鞣花酸高、低剂量组血清HDL-C增加(P<0.01),鞣花酸高剂量组血清TC降低(P<0.05);HE染色观察发现鞣花酸高、低剂量组脂肪细胞直径显著变小,肝组织脂滴空泡明显减少,且呈剂量依赖性。结论:鞣花酸可能通过抑制高脂饮食大鼠的摄食量从而抑制其体重的增加。
Objective: To study the effects of ellagic acid on obesity prevention induced by high-fat diet in rats. Methods: Forty SD male rats were randomly divided into ellagic acid high dose group (high fat diet +30 mg / kg / d ellagic acid, EAH), ellagic acid low dose group (high fat diet +10 (ellagic diet +10.8 mg / kg / d orlistat, O), model control group (high fat diet +10 mL / kg / d 0.5% The rats were randomly divided into two groups: control group (n = 10), control group (n = 10), sodium alginate solution (CMC sodium solution, HFD) and blank control group (normal diet +10 mL / The indexes of organ and fat were calculated and the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL- And liver cell morphology. Results: Compared with model control group, body weight and food intake of ellagic acid in high and low dose groups decreased significantly (P <0.05) and fat coefficient decreased significantly (P <0.001) (P <0.01). Serum TC of high-dose ellagic acid group was lower than that of high-dose ellagic acid group (P <0.05). The diameter of fat cells in high-dose and low- Tissue lipid vacuoles decreased significantly, and in a dose-dependent manner. Conclusion: Ellagic acid may inhibit the increase of body weight by inhibiting the food intake of high-fat diet rats.