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以两个转hpa1Xoo基因的棉花株系T-33和T-35及其出发品种即非转基因棉品种Z35的2~3叶期棉花幼苗为试材,通过无底塑料盆钵菌液浇根接菌法,在0~10 d不同时段测定叶片浸出液电导率。转基因棉T-34在0~10 d间相对电导率呈增加趋势,10 d时增加率达到115.90%;T-33在接菌后0~1 d间的电导率增幅反而略有下降,但3~10 d的增幅均呈现上升趋势,到10 d时达到140.61%;Z35从接菌后0~10 d的电导率增幅均呈现逐渐上升趋势,到10 d时相对电导率增加率达到180.78%。转基因棉到达相对电导率峰值的时间晚于非转基因棉,其峰值也低于非转基因棉;同时两个转基因棉花株系相对电导率的增加率均低于非转基因棉。被黄萎病菌感染后转基因棉的显症时间较非转基因棉出现晚,两个转基因株系的受危害程度也低于非转基因棉。
Two cotton seedlings of cotton plants T-33 and T-35 which transfered hpa1Xoo gene and the non-transgenic cotton variety Z35 were used as test materials, Bacteria method was used to measure the conductivity of leaf extract at different time from 0 to 10 days. The relative conductivity of transgenic cotton T-34 tended to increase from 0 to 10 d, and the increasing rate reached 115.90% on the 10th day. However, the increase of conductivity of T-33 decreased slightly from 0 to 1 d after inoculation, ~ 10 d increased by 140.61% at 10 days. The conductivity of Z35 increased gradually from 0 to 10 days after inoculation, and the relative conductivity increased by 180.78% after 10 days. The peak value of relative conductivity of transgenic cotton reached its peak later than that of non-transgenic cotton, and its peak value was also lower than that of non-transgenic cotton. Meanwhile, the relative conductivity of two transgenic cotton lines was lower than that of non-transgenic cotton. Transgenic cotton infected with Verticillium dahlia showed more obvious symptoms than non-transgenic cotton, and the transgenic plants were also less harmful than non-transgenic cotton.