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伸展蛋白(Extensin)作为一种细胞壁蛋白被认为是与植物的抗病性有关。利用胡萝卜愈伤组织伸展蛋白的抗体,借助于免疫学交叉反应,对大豆下胚轴中伸展蛋白的种类及诱导因素差异引起不同伸展蛋白的合成进行了研究。Western Blotting分析结果表明,0.2MCaCl2可以从大豆下胚轴细胞壁中提取得到一种细胞壁蛋白质组分,这个组分不仅在电泳性质及免疫原性质上与胡萝卜愈伤组织伸展蛋白相同,并且在与细胞壁结合的性质上也相同。真菌诱导物处理的大豆下胚轴中总的伸展蛋白的积累高于受伤处理的大豆下胚轴,并且差异主要是由于与胡萝卜伸展蛋白相同电泳性质蛋白质组分造成的。利用Western Blotting分析和斑点-酶联免疫吸附分析观察不同抗灰斑病品系的大豆下胚轴积累伸展蛋白的速度发现,抗性品系(合丰29和丰收10)的大豆下胚轴可以较迅速积累伸展蛋白(在约24—48小时),而感病品系大豆下胚轴(绥农4)则积累伸展蛋白较晚(约在72小时)。这些结果表明细胞壁伸展蛋白的积累快慢可能是造成大豆对灰斑病表现抗性还是感病的因素之一。
Extensin as a cell wall protein is thought to be involved in plant disease resistance. The use of carrot callus extensin antibody, by means of immunological cross-reaction, the type of epitaxy in soybean hypocotyls and the induction of differences caused by the different stretch protein synthesis were studied. The results of Western Blotting showed that 0.2MCaCl2 could extract a cell wall protein from the cell wall of soybean hypocotyls, which was not only the same as carrot callus extensin in electrophoretic property and immunogenicity, The same is true for the combination. The accumulation of total extensin in fungus inducer-treated soybean hypocotyls was higher than that of injured plants, and the difference was mainly due to the same electrophoretic protein components as carrot extensin. Western Blotting analysis and dot-ELISA analysis of the accumulation of extensin in soybean hypocotyls revealed that the resistance of soybean lines to the hypocotyls of resistant lines (Hefeng 29 and Bumper10) Extensions accumulate (at about 24-48 hours), whereas the susceptible strain soybean hypocotyls (Suinong 4) accumulate late proteins (about 72 hours later). These results indicate that the accumulation of cell wall extensin may be one of the factors that cause the soybean to show resistance to gray spot disease.