论文部分内容阅读
目的 :通过对H1受体基因敲除模型鼠 [(- / - ) (HIKO) ]进行的评价性研究 ,更进一步探索组胺H1受体的生理意义。方法 :对 (- / - )鼠及野生型鼠 (+ / + )进行了 3项评价性实验 :即PCR检测、标记配体 [3H]Pyrilamine的H1受体结合实验及福尔马林疼痛感觉试验 ,并进行了小鼠脑各部位单胺含量的测定。结果 :PCR检测的结果是变异基因为 90 0bp、野生型鼠 (+ / + )基因为12 0 0bp。受体结合实验 (+ / + )鼠与 (- / - )鼠比较其具结合能有显著性差异 (P <0 .0 5 )。福尔马林试验两者比较 ,第二时相 (- / - )鼠的舔、咬注射部位的行为的减少与 (+ / + )鼠比较有显著差异 (P <0 .0 5 )。用高效液相色谱分析仪测定的脑中单胺含量的结果显示 :(- / - )鼠与 (+ / + )鼠比较 ,(- / - )鼠的 5 HT的代谢回转率 (5 HIAA/ 5 HT)在丘脑、大脑皮质、海马、脑干部都有显著地增加 (P <0 .0 5 )。结论 :从 (- / - )鼠首次观察到的疼痛感受性低下及 5 羟色胺含量增高的结果可推测组胺中枢神经系统H1受体与疼痛调控机能有着相关性
OBJECTIVE: To further investigate the physiological significance of histamine H1 receptor through the evaluation of [(- / -) (HIKO)] in H1 receptor knockout mice. METHODS: Three evaluation experiments were performed on (- / -) mice and wild-type mice (+ / +): PCR assay, labeling of ligand [3H] Pyrilamine for H1 receptor binding assay and formalin pain sensation Test, and the determination of monoamine content in all parts of mouse brain. Results: The result of PCR showed that the mutation gene was 90 0 bp and the wild-type mouse (+ / +) gene was 120 kb. There was significant difference (P <0.05) in the binding between receptor binding assay (+ / +) mice and (- / -) mice. In the formalin test, there was a significant difference (P <0. 05) in the licking and bite reduction in the second phase (- / -) mice compared with the (+ / +) mice. The results of monoamine content in the brain measured by a high performance liquid chromatograph showed that the rate of 5 HTIA turnover in the (- / -) mice compared with (+ / +) mice (5 HIAA / 5 HT) increased significantly in thalamus, cerebral cortex, hippocampus and brain stem (P <0.05). CONCLUSIONS: The first hypothesis of pain sensitivity and increased serotonin levels observed in (- / -) mice suggest that there is a correlation between histamine H1 receptors in the central nervous system and pain regulation