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在脑缺氧损伤的研究中,离体海马切片既可排除血脑屏障,又保持原有神经环路,所得实验结果接近在体实验,是研究脑缺氧的理想模型。近几年来国外已有人报道了缺氧时海马脑片诱发电位的变化;但同时用两个脑片比较不同程度的低氧对脑片诱发电位的影响,却少有报道。本实验采用自行设计的双孔脑片电生理实验系统,用不同浓度的低氧混合气造成不同程度的缺氧环境,以探明不同缺氧程度影响脑片电反应的时间曲线,寻求海马脑片缺氧损伤的特征性反应与不可逆损伤发生的临界值。
In the study of hypoxic injury, isolated hippocampal slices not only exclude the blood-brain barrier but also maintain the original neural circuit. The experimental results obtained are close to in vivo experiments and are ideal models for studying hypoxia in the brain. In recent years, foreign countries have been reported changes in hippocampal slices induced by hypoxia; but at the same time with two slices of different degrees of hypoxia on brain evoked potentials, but few reports. In this study, a self-designed double-hole brain electrophysiology experimental system was used. Different concentrations of hypoxia mixed gas resulted in different degrees of hypoxia, in order to find out the time curve of different hypoxic levels affecting brain electrical response, Characteristic Response of Hypoxic Injury and Critical Value of Irreversible Injury.