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目的研究Survivin表达抑制对鼻咽癌细胞增殖和凋亡的影响,探讨Survivin在鼻咽癌中的生物学功能。方法采用脂质体将Survivin特异性SiRNA(small interfering RNA)转入鼻咽癌细胞株5-8F,培养48h后,收集细胞。逆转录-聚合酶链式反应(RT-PCR)、流式细胞仪(flow cytometry,FCM)分别检测Survivin mRNA和蛋白在细胞中表达。流式细胞仪检测细胞凋亡与周期,显微镜下计数检测细胞增殖抑制。结果SiRNA抑制5-8F细胞Survivin表达后,细胞凋亡指数显著高于对照组(P<0.01);S/G1比值显著高于对照组(P<0.01);细胞增殖抑制率也显著高于对照组(P<0.01);脂质体对照组与阴性SiRNA对照组比较,细胞凋亡、周期和增殖差异无显著性(P>0.05)。结论Survivin表达抑制,诱导鼻咽癌细胞凋亡并抑制其增殖。
Objective To investigate the effect of Survivin expression on the proliferation and apoptosis of nasopharyngeal carcinoma cells and to investigate the biological function of Survivin in nasopharyngeal carcinoma. Methods Survivin-specific siRNA (siRNA) was transfected into nasopharyngeal carcinoma cell line 5-8F by liposome and cultured for 48 hours. The expression of Survivin mRNA and protein were detected by RT-PCR and flow cytometry (FCM). Apoptosis and cycle were detected by flow cytometry and cell proliferation inhibition by counting under microscope. Results SiRNA inhibited the expression of Survivin in 5-8F cells significantly (P <0.01), and the ratio of S / G1 was significantly higher than that in control group (P <0.01). The inhibition rate of SiRNA was also significantly higher than that of control (P <0.01). There was no significant difference in apoptosis, cycle and proliferation among the control group and the negative control group (P> 0.05). Conclusion The expression of Survivin inhibits the apoptosis of nasopharyngeal carcinoma cells and inhibits its proliferation.