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大蒜紫斑病菌是一种严重危害大蒜生产的真菌性病害,是中国大蒜出口中需要检疫的一种病害。根据1,3,8-三羟基萘还原酶基因(Brn1)的部分序列,设计出一对大蒜紫斑病菌特异性引物AP4/CTU,该引物特异性好,能专一扩增出480 bp电泳条带,而供试大蒜上的其他病害、不同属的真菌及空白对照均无扩增条带,建立了大蒜紫斑病菌的PCR鉴定方法。该方法灵敏度较高,DNA检测灵敏度为4 ng。该方法快速简便,适用于出入境检验检疫及大蒜健康检测领域。
Garcinia hymenopus is a serious fungal disease that endangers the production of garlic and is a disease that requires quarantine in China’s exports of garlic. According to the partial sequence of the 1,3,8-trihydroxynaphthalene reductase gene (Brn1), a pair of specific primers of the genus V. hygroscopicus, AP4 / CTU, was designed. The primer has good specificity and can specifically amplify a 480 bp electrophoresis strip However, there was no amplified bands in other diseases of the tested garlic, fungi in different genus and blank control, and PCR identification method of V. versicolor was established. This method is more sensitive and has a DNA detection sensitivity of 4 ng. The method is fast and easy, suitable for exit-entry inspection and quarantine and garlic health testing.