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目的观察北太平洋鱿鱼墨多糖对化疗小鼠肠道黏膜损伤的保护作用,并初步探讨其作用机制。方法连续给小鼠灌胃鱿鱼墨多糖28 d,d 26、27连续两天腹腔注射环磷酰胺建立化疗小鼠模型,d 29脱颈椎处死。取空肠组织进行石蜡切片,HE染色,统计绒毛高度、隐窝深度及绒毛高度与隐窝深度比值(V/C)。取肠组织匀浆进行蛋白质电泳,考马斯亮蓝染色观察特异性表达的蛋白质。取特异性表达蛋白质进行质谱分析,鉴定蛋白质种类。取肠匀浆上清检测丙二醛(MDA)和超氧化物歧化酶(SOD)活性变化。结果 (1)鱿鱼墨多糖中、高剂量组小肠绒毛高度明显高于环磷酰胺模型组(P<0.01);鱿鱼墨多糖各剂量组小肠隐窝深度均明显低于环磷酰胺模型组(P<0.01);鱿鱼墨多糖各剂量组V/C比值均明显高于环磷酰胺模型组(P<0.01)。(2)质谱结果显示,特异性表达的蛋白条带之一为谷胱甘肽巯基转移酶ω-1。(3)模型组SOD活性降低,MDA含量增高,而鱿鱼墨多糖可以提高SOD活性,降低MDA含量。结论鱿鱼墨多糖具有保护小鼠肠道黏膜的作用,预防环磷酰胺所致的消化道不良反应。上述作用与鱿鱼墨多糖能够提高小鼠肠道黏膜的抗氧化能力有关。
Objective To observe the protective effect of Mexican squid polysaccharides on intestinal mucosal injury in mice and to explore its mechanism. Methods Mice were given squid ink polysaccharides continuously for 28 days. On days 26 and 27, cyclophosphamide was intraperitoneally injected to establish a mouse model of chemotherapy. The jejunal tissue was taken for paraffin section and stained with hematoxylin and eosin (HE). The villus height, crypt depth, villus height to crypt depth ratio (V / C) were calculated. Gut tissue homogenates were subjected to protein electrophoresis, Coomassie brilliant blue staining to observe specifically expressed proteins. The specific expression of the protein to take mass spectrometry, identification of protein species. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in intestinal homogenate were measured. Results (1) The intestinal villus height in medium and high dose squid polysaccharides group was significantly higher than that in cyclophosphamide model group (P <0.01). The depth of small intestine crypts in squid polysaccharides group was significantly lower than that in cyclophosphamide model group <0.01). The V / C ratio of squid polysaccharide in each dose group was significantly higher than that in cyclophosphamide model group (P <0.01). (2) Mass spectrometry showed that one of the specifically expressed protein bands was glutathione S-transferase ω-1. (3) SOD activity in model group decreased and MDA content increased, while squid polysaccharides increased SOD activity and decreased MDA content. Conclusion Squid polysaccharides have the function of protecting intestinal mucosa of mice and preventing the gastrointestinal side effects caused by cyclophosphamide. The above effects and squid polysaccharides polysaccharide can improve the mouse intestinal mucosal antioxidant capacity.