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研究化学物引起染色体畸变,是快速筛选环境致突变原和致癌原的有用方法。体外培养哺乳动物细胞的研究表明,某些致癌多环烃(CPH)代谢活化后有致染色体断裂作用。本文所用中国仓鼠细胞V_(79),常规培养在10ml Eagle’s最低基本培养基(MEM)中,这种培养基中有10%小牛血清和抗菌素。于37℃,95%空气和5%CO_2条件下孵育。在5
Research chemicals cause chromosomal aberrations, is a useful method for rapid screening of mutagens and carcinogens in the environment. In vitro studies of mammalian cells have shown that some of the carcinogenic polycyclic hydrocarbons (CPH) undergo metabolic chromosomal cleavage upon activation. As used herein, Chinese hamster V_ (79) cells are routinely cultured in 10 ml Eagle’s Minimum Essential Medium (MEM) with 10% bovine serum and antibiotics in this medium. Incubate at 37 ° C, 95% air and 5% CO 2. At 5