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近年来HPLC采用光电二极管阵列检测器,使色谱峰的鉴定有了明显改进.它可同时记录各种不同波长所测得的色谱图,将层析洗脱出的每一种化合物进行UV测量.该法适用于复杂混合物的分析.作者使用HPLC与UV联用仪快速测定植物提取中的多酚类物质,采用柱后衍生系统,将位移试剂加入到洗脱液中,所得UV图谱比不加位移试剂时有明显改进.在洗脱过程中所
In recent years HPLC has adopted the photodiode array detector, the identification of the chromatographic peak has been significantly improved.It can simultaneously record the chromatograms measured at different wavelengths, the chromatographic eluted each compound for UV measurement. The method is suitable for the analysis of complex mixtures.The author uses HPLC and UV combined with rapid determination of plant extracts of polyphenols, the use of post-column derivatization system, the displacement reagent was added to the eluate, the resulting UV spectrum than not Displacement reagents significantly improved in the elution process