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本文研究了抗叶锈性不同的小麦品种和毒力不同的叶锈菌小种相互作用过程中过氧化物酶(POD)同工酶谱的变化。结果表明:(1)不亲和组合在接种后48小时出现新酶带3,酶带4活性明显增强,120小时后又出现新酶带9。但亲和组合没有新酶带出现,而在接种后96小时酶带4活性也明显增强;(2)慢锈品种在接种后120小时以前,酶带4活性稍有增强,在120小时后其活性骤然增强。这可能是慢锈品种的特点。如果这些特点在更多的品种上得到验证,则POD同工酶谱分析有可能作为鉴定品种抗叶锈性和区分感病或慢锈品种的一种生理生化指标。
In this paper, the changes of peroxidase (POD) isozymes during the interaction of wheat cultivars with different leaf rust resistances and the virulence of different leaf rust races were studied. The results showed that: (1) The new enzyme band 3 appeared 48 hours after inoculation in the unaffinity group, the activity of the enzyme band 4 was obviously enhanced, and the new enzyme band appeared after 120 hours. However, there was no new band appeared in the affinity combination, but the activity of the band 4 was significantly increased at 96 hours after inoculation. (2) The activity of the band 4 was slightly increased 120 hours after inoculation, Suddenly increased activity. This may be a feature of slow-rusting varieties. If these characteristics are validated in more varieties, POD isozyme mapping may be used as a physiological and biochemical indicator to identify leaf rust resistance and differentiate susceptible or slow-rusting varieties.