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目的:建立同时测定大鼠血浆中绿原酸和栀子苷浓度的方法。方法:以甲醇为溶剂,采用蛋白沉淀法处理血浆,采用高效液相色谱(HPLC)法测定药物浓度。色谱柱为大连依利特C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%甲酸(14∶86),内标为紫丁香苷。内标、绿原酸、栀子苷的检测波长分别为266 nm(0~5.9 min)、328 nm(6.0~7.9 min)和234 nm(8.0~12min)。结果:绿原酸和栀子苷分别在1.625~104.0,1.925~123.2μg·mL~(-1)内线性关系良好,回收率分别在92.25%~99.36%和91.96%~98.57%范围内,RSD均低于6%。结论:该方法简便、准确,可用于同时测定绿原酸和栀子苷的血药浓度。
Objective: To establish a method for simultaneous determination of chlorogenic acid and geniposide in rat plasma. Methods: The plasma was treated with methanol as the solvent and the protein concentration was determined by high performance liquid chromatography (HPLC). The column was Dalian Elixel C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid (14:86) and the internal standard was syringin. The detection wavelength of internal standard, chlorogenic acid and geniposide were 266 nm (0-59 min), 328 nm (6.0-7.9 min) and 234 nm (8.0-12 min), respectively. Results: The linear relationship between chlorogenic acid and geniposide was 1.625 ~ 104.0 and 1.925 ~ 123.2 μg · mL ~ (-1), respectively. The recoveries were in the range of 92.25% -99.36% and 91.96% -98.57% All less than 6%. Conclusion: The method is simple and accurate and can be used for the simultaneous determination of plasma concentrations of chlorogenic acid and geniposide.