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目的研究表皮生长因子（ｅｎｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＥＧＦ）、神经降压素（ｎｅｕｒｏｔｅｎｓｉｎ， ＮＴ）对四氯化碳损伤的原代培养肝细胞Ｃａ~（２＋）及膜流动性的影响。方法以Ｆｕｒａ－２／ＡＭ和ＤＰＨ荧光探针测定肝细胞内游离钙和膜流动性，观察ＥＧＦ、ＮＴ对四氯化碳损伤后肝细胞这两个指标的影响。结果ＥＧＦ、ＮＴ明显对抗肝细胞四氯化碳损伤后胞内［Ｃａ~（２＋）］ ｉ的增高和膜流动性的降低。结论 ＥＧＦ、 ＮＴ有维持肝细胞膜流动性和胞浆钙稳态的作用。
Objective To investigate the effect of enotaxial growth factor (EGF) and neurotensin (NT) on Ca 2+ and membrane fluidity in primary cultured hepatocytes injured by carbon tetrachloride. Methods The intracellular free calcium and membrane fluidity were measured with Fura-2 / AM and DPH fluorescent probes. The effects of EGF and NT on the two indexes of liver cells after carbon tetrachloride injury were observed. Results EGF and NT obviously antagonized the increase of intracellular [Ca2 +] i and the decrease of membrane fluidity after carbon tetrachloride injury in hepatocytes. Conclusion EGF and NT can maintain the membrane fluidity and cytoplasmic calcium homeostasis in hepatocytes.