论文部分内容阅读
目的:鉴定中药飞龙掌血体外抗流感病毒(A/PR/8/34H1N1)活性。方法:用四唑氮化合物(MTS)测定流感病毒诱导细胞病变(CPE)方法,初筛200余种中草药提取物抗A型流感病毒活性。复筛中样品的抗病毒活性被进一步用荧光定量聚合酶链反应(PCR)测试方法验证。结果:飞龙掌血在MTS方法和定量PCR中均显示很强的抗H1N1型流感病毒活性,半数有效剂量(EC50)在MTS方法和定量PCR中分别为4.7,0.9mg·L-1。毒性实验显示半数细胞毒剂量(CC50)为187.2mg·L-1,选择指数(SI)在定量PCR测试中大于206。药物作用时间实验显示飞龙掌血与病毒同时加入细胞可获得最佳抗病毒效果,病毒感染前、后24h加入飞龙掌血仍然具有一定抗病毒作用。结论:飞龙掌血可作为抗流感病毒候选治疗药物。
OBJECTIVE: To identify the anti-influenza virus (A/PR/8/34H1N1) activity of Feilong palm in vitro. METHODS: Influenza virus-induced cytopathic effect (CPE) was measured with tetrazolium (MTS) and the activity of more than 200 Chinese herbal extracts against influenza A virus was screened. The antiviral activity of the rescreened samples was further verified by a fluorescent quantitative polymerase chain reaction (PCR) test method. RESULTS: M. pterosaurs showed strong anti-H1N1 influenza virus activity in MTS assay and quantitative PCR. The half effective dose (EC50) was 4.7 and 0.9 mg·L-1 in MTS assay and quantitative PCR, respectively. Toxicity experiments showed that the half cytotoxic dose (CC50) was 187.2 mg·L-1 and the selection index (SI) was greater than 206 in the quantitative PCR test. The time of action of the drug showed that the best antiviral effect was obtained by adding the cells of both the pterosaur blood and the virus simultaneously. The addition of pterosaurs blood before and after the virus infection still had some antiviral effect. Conclusion: Feilong palm blood can be used as a candidate for treatment against influenza virus.