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为了简化丝素微球的制备方法及保证丝素微球作为药物缓释载体的安全性与结构稳定性,以丝素蛋白为原材料,采用自组装方法并调节丝素蛋白溶液浓度、冷冻温度、异丙醇添加量等条件,制备粒径在0.4~1.3μm之间的丝素微球。扫描电子显微镜(SEM)下观察制备的丝素微球呈规则的球状结构,球面光滑,粒径分布较为均匀;采用红外光谱仪(FT-IR)和热重分析仪(DTG)测试自组装后丝素微球的分子构象已发生转化,β-折叠结构含量明显增加,并具有较好的热稳定性。研究结果表明,丝素蛋白溶液浓度、冷冻温度、异丙醇添加量对丝素微球的形态结构有影响,可以通过自组装方法制备出粒径可控,结构和性能稳定的丝素微球,并有望开发为药物缓释载体。
In order to simplify the preparation method of silk fibroin microspheres and to ensure the safety and structural stability of silk fibroin microspheres as drug delivery carrier, the silk fibroin was used as raw material and self-assembly method was used to adjust the silk fibroin solution concentration, freezing temperature, Isopropanol and other conditions to prepare a diameter of 0.4 ~ 1.3μm silk fibroin microspheres. Under the scanning electron microscope (SEM) observation, the prepared silk fibroin microspheres showed a regular spherical structure with a smooth spherical surface and a uniform particle size distribution. The microstructure of the fibroin microspheres was analyzed by FT-IR and DTG. The molecular conformation of the microspheres has been transformed, the content of β-sheet structure increased significantly, and has good thermal stability. The results showed that the concentration of silk fibroin solution, freezing temperature, the amount of isopropanol on the morphology of silk fibroin microspheres have an impact, can be prepared by self-assembly method to control the size, structure and properties of stable fibroin microspheres , And is expected to be developed as a sustained-release drug carrier.