一株惊纹地老虎幼虫颗粒体病毒的研究

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1978年春,在新疆博湖从惊纹地老虎Agrotis exclamationis(Linné)(鳞翅目:夜蛾科)幼虫中发现一株病毒“Ae—02”,病毒颗粒体为卵形或卵圆形,大小为374~780×312~450nm,平均为578×412nm;病毒粒子杆状,两端钝圆,不弯曲或稍弯曲,大小为350~450×40~65nm,平均为395×50nm。该病毒对惊纹地老虎和黄地老虎A.segetum(Schiff.)幼虫致病力强。在日平均温度为24.2℃时,对1日龄惊纹地老虎幼虫感病19天后死亡率达96~100%;25天后10日龄幼虫死亡率达84~92%;对15日龄幼虫死亡率为73.96~80.96%。惊纹地老虎病毒“Ae—02”毒株,属杆状病毒科Baculoviridae,杆状病毒属Baculovirus,“B”亚组,定名为惊纹地老虎颗粒体病毒Agrotis excula-mationis granulosis virus,俗名鸣夜蛾颗粒体病毒,拉丁学名为Baculovirusexclamationis“B”,简称AeGV。 In the spring of 1978, a strain of virus “Ae-02” was found in larvae of Agrotis exclamationis (Linné) (Lepidoptera: Noctuidae) in Bohu Lake, Xinjiang. The virus particles were oval or oval in size, Was 374-780 × 312-450 nm with an average of 578 × 412 nm. The virus particles were rod-like and blunt round at both ends with no bending or slight bending. The size of the virus particles was 350-450 × 40-65 nm with an average of 395 × 50 nm. The virus is particularly virulent to the larvae of A. segetum (Schiff.). At day average temperature of 24.2 ℃, the mortality rate of 96 days to 1 day old tiger larvae was 96 to 100% after 19 days. The death rate of 10-day-old larvae reached 84-92% after 25 days. The mortality of 15-day-old larvae The rate was 73.96 ~ 80.96%. The virus named “Ae-02” was named Baculoviridae and Baculovirus of baculovirus family “B”, named Agrotis excula-mationis granulosis virus Worm granulovirus, Latin scientific name Baculovirusexclamationis “B”, referred to as AeGV.
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