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目的:观察龙胆泻肝汤提取物体外对白念珠菌生物膜形成影响,探讨其可能的作用机制。方法:采用XTT法和微量稀释法筛选龙胆泻肝汤各提取部位的抗菌活性并检测各提取物对白念珠菌生物膜的抑制作用。采用实时荧光定量PCR法检测其黏附相关基因ALS1和菌丝形成基因SUN41的表达量。结果:龙胆泻肝汤用石油醚、水、正丁醇、甲醇及乙酸乙酯提取部位对白念珠菌浮游菌的MIC分别为>1 000,>1 000,>1 000,125,125 mg·L-1;对白念珠菌生物膜的SMIC50分别为>1 000,>1 000,>1 000,500,500 mg·L-1;SMIC80分别为>1 000,>1 000,>1 000,>1 000,1 000 mg·L-1;1 000 mg·L-1的乙酸乙酯提取物能明显抑制黏附相关基因ALS1和菌丝形成基因SUN41的表达。结论:龙胆泻肝汤抗白念珠菌生物膜的活性部位为乙酸乙酯提取部位。
Objective: To observe the effect of extract of Longdan Xiegan decoction on Candida albicans biofilm in vitro and to explore its possible mechanism. Methods: The antimicrobial activity of Longdan Xiegan decoction was screened by XTT method and microdilution method. The inhibitory effect of each extract on Candida albicans biofilm was also tested. Real-time quantitative PCR was used to detect the expression of adhesion-related gene ALS1 and mycelium formation gene SUN41. Results: The MICs of Candida albicans were> 1 000,> 1 000,> 1 000, 125 and 125 mg · L -1, respectively, for the extracts from Longdan Xiegan Decoction with petroleum ether, water, n-butanol, methanol and ethyl acetate. The MICs of Candida albicans biofilm were> 1000,> 1000,> 1,500, 500 and 500 mg · L-1, respectively; SMIC80 were> 1000,> 1 000,> 1 000,> 1 000 and 1 000 mg L -1; 1 000 mg · L -1 ethyl acetate extract can significantly inhibit the expression of the adhesion-related gene ALS1 and the mycelium forming gene SUN41. Conclusion: The active site of Candida albicans biofilm of Longdan Xiegan decoction is ethyl acetate extract.