论文部分内容阅读
目的探讨血小板源性生长因子(PDGF)-BB及肝细胞生长因子(HGF)对体外培养的人子宫内膜腺上皮细胞增殖的影响及其最佳剂量,以及PDGF-BB及HGF对子宫内膜腺上皮细胞整合素αⅤβ3表达的影响。方法 2009年10月至2010年12月在中国医科大学附属盛京医院分离人子宫内膜腺上皮细胞及基质细胞,分别在体外培养。用特异性抗体鉴定细胞纯度,用MTT法检测PDGF-BB及HGF对子宫内膜腺上皮细胞增殖的影响,并用免疫组化方法检测子宫内膜腺上皮细胞整合素αⅤβ3的表达。结果分离的子宫内膜腺上皮细胞及基质细胞纯度高,特异性抗体鉴定细胞纯度分别为95%和97%。不同质量浓度的PDGF-BB及HGF作用后吸光度值均增加。PDGF-BB组吸光度值随着质量浓度的升高而升高,HGF组在质量浓度为20μg/L时为峰值。质量浓度为10μg/L的PDGF及20μg/L的HGF作用后子宫内膜腺上皮细胞整合素αⅤβ3的表达最强。结论 PDGF-BB及HGF均能促进体外培养的人子宫内膜腺上皮细胞增殖,并能促进子宫内膜腺上皮细胞整合素αⅤβ3的表达。
Objective To investigate the effects of PDGF-BB and hepatocyte growth factor (HGF) on the proliferation of human endometrial glandular epithelial cells cultured in vitro and the optimal dosage of PDGF-BB and HGF for endometrial Effect of integrin αⅤβ3 expression in glandular epithelial cells. Methods From October 2009 to December 2010, human endometrial glandular epithelial cells and stromal cells were isolated from Shengjing Hospital affiliated to China Medical University and cultured in vitro. The purity of cells was identified by specific antibodies. The effects of PDGF-BB and HGF on the proliferation of endometrial glandular epithelial cells were detected by MTT assay. The expression of integrin αⅤβ3 in endometrial glandular epithelial cells was detected by immunohistochemistry. Results The isolated endometrial glandular epithelial cells and stromal cells were highly pure. The purity of the specific antibody-specific cells was 95% and 97%, respectively. The absorbance values of PDGF-BB and HGF with different mass concentrations increased. The absorbance value of PDGF-BB group increased with the increase of concentration, while the peak value of HGF group was reached at the concentration of 20μg / L. The expression of integrin αVβ3 in endometrial glandular epithelial cells was the strongest when the concentration of PDGF was 10μg / L and the concentration of HGF was 20μg / L. Conclusion Both PDGF-BB and HGF can promote the proliferation of human endometrial glandular epithelial cells cultured in vitro and promote the expression of integrin αⅤβ3 in endometrial glandular epithelial cells.