论文部分内容阅读
目的：阐明肺炎时一氧化氮（ Ｎ Ｏ）和肿瘤坏死因子（ Ｔ Ｎ Ｆ）的变化。方法：采用比色法、双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ 法分别测定４１例肺炎患儿及２５例正常健康儿血清 Ｎ Ｏ、 Ｔ Ｎ Ｆ水平；并研究了 Ｎ Ｏ 与 Ｔ Ｎ Ｆ的关系。结果：急性期肺炎患儿的 Ｎ Ｏ、 Ｔ Ｎ Ｆ 较对照组、恢复期患儿明显升高；重症组较轻症组明显升高；而且 Ｎ Ｏ 与 Ｔ Ｎ Ｆ呈高度正相关。结论：血清 Ｎ Ｏ、 Ｔ Ｎ Ｆ 水平与肺炎的发生、发展和病情严重程度有关。
Objective: To clarify the changes of nitric oxide (N O) and tumor necrosis factor (T N F) in patients with pneumonia. Methods: The serum levels of N O, T N F in 41 children with pneumonia and 25 healthy children were measured by colorimetric method and double antibody sandwich ELISA method. The relationship between N O and T N F was also studied. Results: The levels of N O, T N F in children with acute pneumonia were significantly higher than those in control group and convalescent group, and those in severe group were significantly higher than those in mild group. There was a positive correlation between N O and T N F. Conclusion: The serum levels of N O, T N F are related to the occurrence, development and severity of pneumonia.