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为建立鉴别致病性结核杆菌感染和卡介苗接种产生的IgG抗体,分别用结核杆菌的混合抗原TB1、TB2和PPD包被,以结核病患者血清为阳性对照,以PPD试验阴性健康人血清作为阴性对照,建立了TB1-ELISA、TB2-ELISA、PPD-ELISA三种检测结核杆菌抗体的方法。20份阳性标本检测结果显示,TB1-ELISA、TB2-ELISA、PPD-ELISA的阳性检出率分别为45%、50%、95%,100份健康人血清标本检测结果显示,TB1-ELISA、TB2-ELISA、PPD-ELISA的符合率为93%、90%、68%。研究工作为研发致病性结核杆菌感染IgG抗体检测试剂奠定了一定基础。
To establish the identification of pathogenic Mycobacterium tuberculosis infection and BCG vaccination generated IgG antibodies were TB Mycobacterium tuberculosis mixed TB1, TB2 and PPD coating to tuberculosis patients serum as a positive control PPD test negative healthy human serum as a negative control , Established TB1-ELISA, TB2-ELISA, PPD-ELISA detection of Mycobacterium tuberculosis antibody. The positive detection rates of TB1-ELISA, TB2-ELISA and PPD-ELISA were 45%, 50%, 95% respectively in 20 positive samples. The results of 100 serum samples from healthy volunteers showed that TB1-ELISA, TB2 -ELISA, PPD-ELISA coincidence rate of 93%, 90%, 68%. Research work for the development of pathogenic Mycobacterium tuberculosis infection IgG antibody detection reagents and laid a solid foundation.