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目的探讨吗啡对人类免疫缺陷病毒-1(HIV-1)在MT2细胞中复制的影响。方法将MT2细胞按单纯随机分组的原则分为吗啡处理组、吗啡+纳洛酮处理组、纳洛酮处理组和病毒对照组,先用浓度为10-8mol/L的纳洛酮预处理吗啡+纳洛酮处理组和纳洛酮处理组0.5 h,再用10-12、10-10和10-8mol/L 3个浓度的吗啡处理吗啡+纳洛酮处理组和吗啡处理组24 h,然后每组细胞加入HIV-1感染MT2细胞,并分别于感染的第3、4、5和6 d取培养上清测定HIV-1 p24抗原表达。结果 HIV-1感染MT2细胞第3、4、5、6 d,10-12、10-10和10-8mol/L 3个浓度吗啡处理组的HIV-1 p24抗原表达均高于病毒对照组,差异均有统计学意义(P<0.01);吗啡+纳洛酮处理组、纳洛酮处理组与病毒对照组HIV-1 p24抗原表达比较,差异均无统计学意义(P>0.05);第3、4、5、6 d,3个浓度吗啡处理组的HIV-1 p24抗原表达量比对照组增加的倍数比较,差异均有统计学意义(P<0.05);10-12、10-10和10-8mol/L 3个浓度吗啡处理组在不同时间HIV-1 p24抗原表达量比对照组增加倍数比较,3个吗啡处理组的HIV-1 p24抗原表达量比对照组增加倍数均随着感染时间的延长而呈现递增的趋势(P<0.05)。结论吗啡能够促进HIV-1在MT2细胞和内的复制,并且随感染时间的延长呈现递增趋势;吗啡促进HIV-1复制的作用可被阿片受体阻滞剂纳洛酮阻断。
Objective To investigate the effect of morphine on the replication of human immunodeficiency virus-1 (HIV-1) in MT2 cells. Methods MT2 cells were divided into morphine group, morphine + naloxone group, naloxone group and virus control group according to the principle of simple randomization. Morphine was pretreated with naloxone at the concentration of 10-8mol / L + Naloxone treatment group and naloxone treatment group 0.5 h, then morphine + naloxone treatment group and morphine treatment group at 3, 10-12, 10-10 and 10-8 mol / L for 24 h, Each group of cells was then infected with HIV-1 to infect MT2 cells. The culture supernatants were harvested at days 3, 4, 5 and 6 of infection to determine the expression of HIV-1 p24 antigen. Results The HIV-1 p24 antigen expression of HIV-1 infected MT2 cells at 3, 4, 5, 6 d, 10-12, 10-10 and 10-8 mol / L morphine treatment was higher than that of the virus control group, (P <0.01). There was no significant difference in the expression of HIV-1 p24 antigen between morphine + naloxone treatment group and naloxone treatment group and virus control group (P> 0.05). The difference was statistically significant The expression levels of HIV-1 p24 antigen in morphine treated groups at 3, 4, 5, 6 and 6 days were significantly higher than those in control group (P <0.05) Compared with the control group, the expression levels of HIV-1 p24 antigen at different time points in 10-8mol / L morphine treatment group were significantly higher than those in the control group Infection time showed an increasing trend (P <0.05). Conclusion Morphine can promote the replication of HIV-1 in MT2 cells and in vivo, and it shows an increasing trend with the extension of infection time. The effect of morphine on HIV-1 replication can be blocked by naloxone, an opioid receptor blocker.