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目的探讨是左旋-1-甲基色氨酸(1-L-MT)还是右旋-1-甲基色氨酸(1-D-MT)在小鼠母胎界面诱发母胎免疫耐受失衡。方法雌性BALB/c小鼠与雄性C57BL/6J小鼠交配后,自妊娠第6.5天起分别给予1-L-MT、1-D-MT、有机溶剂,共10 d,于妊娠第16.5天处死孕鼠,应用高效液相色谱法检测胎盘组织中哚胺2,3-二氧化酶(IDO)活性,流式细胞技术检测蜕膜组织中IFN-γ/IL-4比率,并对比妊娠结局。结果 1-L-MT给药组胎盘组织中IDO活性明显低于1-D-MT组及对照组,而1-D-MT组与对照组间无统计学差异;蜕膜组织中IFN-γ/IL-4比率高于1-D-MT组及对照组,而1-D-MT组与对照组间无统计学差异;胎鼠数量及体质量明显低于1-D-MT组及对照组,而1-D-MT组与对照组间无统计学差异。结论 1-L-MT而非1-D-MT通过抑制IDO活性在小鼠母胎界面诱发母胎免疫耐受失衡。
Objective To investigate the imbalance of immunotolerance induced by maternal-fetal interface induced by L-1-methyltryptophan (1-L-MT) or dextran-1-methyltryptophan (1-D-MT) Methods Female BALB / c mice were mated with male C57BL / 6J mice, and 1-L-MT, 1-D-MT and organic solvents were given for 10 days from the 6.5th day of gestation Pregnant rats, the activity of LDH in placenta was detected by HPLC, the ratio of IFN-γ / IL-4 in decidua was detected by flow cytometry, and the pregnancy outcome was compared. Results The IDO activity of placental tissue in 1-L-MT group was significantly lower than that in 1-D-MT group and control group, but there was no significant difference between 1-D-MT group and control group / IL-4 ratio was higher than that of 1-D-MT group and control group, but there was no significant difference between 1-D-MT group and control group; the number of fetal rats and body weight were significantly lower than that of 1-D-MT group and control group Group, while there was no significant difference between 1-D-MT group and control group. Conclusions 1-L-MT, but not 1-D-MT, induces imbalance in maternal immunotolerance at the mouse maternal-fetal interface by inhibiting IDO activity.