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目的:测定龟参口服液中人参皂苷Rg1、Re和Rb1的含量。方法:高效液相色谱法,以十八烷基硅烷键合硅胶为填充剂(250mm×4.6mm,5μm);乙腈-0.4%磷酸溶液为流动相;流速为0.7mL/min;检测波长为203nm;柱温30℃。结果:人参皂苷Rg1在0.72～4.32μg,Re在0.60～3.60μg,Rb1在2.76～16.56μg范围内峰面积与其浓度呈较好的线性关系,平均加样回收率分别为99.30%、99.08%、98.86%,RSD分别为1.09%、1.17%、0.41%。结论:本方法简便、快速、准确,可作为样品的定量方法。
Objective: To determine the contents of ginsenosides Rg1, Re and Rb1 in Tuishen oral liquid. Methods: High performance liquid chromatography with octadecylsilane bonded silica as filler (250mm × 4.6mm, 5μm), acetonitrile-0.4% phosphoric acid as mobile phase, flow rate of 0.7mL / min, detection wavelength of 203nm ; Column temperature 30 ℃. Results: The peak area of ginsenoside Rg1 was 0.72 ~ 4.32μg, Re was 0.60 ~ 3.60μg, Rb1 was 2.76 ~ 16.56μg. The average recoveries were 99.30%, 99.08%, respectively. 98.86%, RSD respectively 1.09%, 1.17%, 0.41%. Conclusion: The method is simple, rapid and accurate and can be used as a quantitative method for samples.