论文部分内容阅读
目的探讨咪喹莫特的免疫调节机制。方法ELISA检测经咪喹莫特灌胃(30mg/kg)后不同时间点的BALB/c小鼠血清干扰素α(IFN-α)、白介素12(IL-12)、干扰素γ(IFN-γ)、白介素4(IL-4)浓度的变化。ELISA检测喂食咪喹莫特的BALB/c小鼠脾细胞在与0.25μg/mL刀豆素A共孵育时分泌IFN-γ、IL-4的能力。结果咪喹莫特体内诱导BALB/c小鼠生成IFN-α、IL-12,在诱导后2h达高峰;咪喹莫特体内无诱导BALB/c小鼠生成IFN-γ、IL-4的作用;接受咪喹莫特灌胃的BALB/c小鼠脾细胞体外受ConA诱导时,分泌IFN-γ和IL-12的能力增强。结论咪喹莫特诱导BALB/c小鼠分泌IFN-α、IL-12等前炎症因子,并由此促进机体获得性免疫。
Objective To investigate the immunomodulatory mechanism of imiquimod. Methods Serum levels of IFN-α, IL-12 and IFN-γ in BALB / c mice at different time points after intragastric administration of Imiquimod (30 mg / kg) ), Interleukin 4 (IL-4) concentration changes. ELISA was used to test the ability of the imiquimod-infused BALB / c mouse splenocytes to secrete IFN-γ and IL-4 when co-incubated with 0.25 μg / mL concanavalin A. Results Imiquimod induced IFN-α and IL-12 production in BALB / c mice in vitro and peaked at 2h after induction. Imiquimod did not induce IFN-γ and IL-4 production in BALB / c mice in vivo The ability of IFN-γ and IL-12 secreting IFN-γ and IL-12 in BALB / c mouse spleen cells fed with imiquimod was enhanced by ConA. Conclusions Imiquimod induces the secretion of proinflammatory cytokines such as IFN-α and IL-12 in BALB / c mice and thus promotes adaptive immunity.