论文部分内容阅读
建立了用反相高效液相色谱-紫外检测法同时检测人尿中6种多环芳烃羟基代谢产物的新方法。采用DiamonsilTMC18(5μm,150 nm×4.6 nm)色谱柱分离,以V(甲醇)∶V(乙酸钠缓冲液)=66∶34为流动相,在波长280 nm检测,流速为0.95 mL/min。结果表明,当9-羟基菲、α-萘酚、β-萘酚、1-羟基芘、2-羟基芴和9-羟基芴的摩尔浓度分别为0.105~500.0μmol/L,0.106~135.0μmol/L,0.120~135.0μmol/L,0.085~50.00μmol/L,0.094~200.0μmol/L和0.101~200.0μmol/L时,峰面积与质量浓度呈很好的线性关系,相对标准偏差分别为2.5%~4.4%,2.1%~4.3%,2.3%~3.2%,1.7%~3.9%,1.9%~2.6%和1.8%~3.5%(n=6),平均回收率分别为92.6%,100.4%,101.0%,91.0%,106.7%和95.5%。方法准确可靠,用于尿样测定,结果满意。
A new method for the simultaneous determination of 6 hydroxycyclic aromatic hydrocarbons metabolites in human urine by reversed-phase high performance liquid chromatography-ultraviolet detection was established. The separation was performed on a Diamonsil TMC18 (5 μm, 150 nm × 4.6 nm) column using V (methanol): V (sodium acetate buffer) = 66:34 as mobile phase at a wavelength of 280 nm with a flow rate of 0.95 mL / min. The results showed that when the molar concentrations of 9-hydroxy phenanthrene, α-naphthol, β-naphthol, 1-hydroxypyrene, 2-hydroxyfluorene and 9-hydroxyfluorene were 0.105-500.0μmol / L, 0.106-135.0μmol / L, 0.120 ~ 135.0μmol / L, 0.085 ~ 50.00μmol / L, 0.094 ~ 200.0μmol / L and 0.101 ~ 200.0μmol / L, the peak area and mass concentration showed a good linear relationship with relative standard deviations of 2.5% The average recoveries were 92.6%, 100.4%, respectively. The average recoveries were 4.4%, 2.1% -4.3%, 2.3% -3.2%, 1.7% -3.9%, 1.9% -2.6% and 1.8% -3.5% 101.0%, 91.0%, 106.7% and 95.5%. The method is accurate and reliable for the determination of urine samples with satisfactory results.