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目的：观察维拉帕米对人单核巨噬细胞ＤＮＡ、ＲＮＡ及蛋白质合成过程的影响。方法：维拉帕米与人单核巨噬细胞孵育２４ｈ或４ｄ，测定细胞３Ｈ－ＴｄＲ、３Ｈ－ＵＲ、３Ｈ－Ｌｅｕ掺入人单核巨噬细胞状态及细胞内蛋白质含量。结果：维拉帕米在低浓度时对ＤＮＡ、ＲＮＡ合成有抑制作用，呈浓度依赖性；高浓度时作用明显，对ＲＮＡ作用较ＤＮＡ显著（Ｐ＜０．０５）；对蛋白质合成在高浓度时有抑制作用。维拉帕米与成熟过程中人单核巨噬细胞作用４ｄ，细胞蛋白含量显著降低（Ｐ＜０．０５），并呈浓度依赖性。结论：此作用可能为维拉帕米抑制细胞生长及动脉粥样斑块形成逆转心肌肥厚的机制之一。
Objective: To observe the effect of verapamil on the DNA, RNA and protein synthesis of human monocyte-macrophage. Methods: Verapamil was incubated with human monocyte-macrophages for 24h or 4d, and the 3H-TdR, 3H-UR and 3H-Leu incorporation into human monocyte-macrophage and the content of intracellular protein were measured. Results: Verapamil had inhibitory effect on DNA and RNA synthesis in a concentration-dependent manner at low concentration. The effect of verapamil at higher concentration was more significant than that of DNA at the high concentration (P <0.05) When the inhibitory effect. Verapamil and human monocyte-derived macrophages in maturation process for 4 days, cell protein content was significantly reduced (P <0.05), and in a concentration-dependent manner. CONCLUSION: This effect may be one of the mechanisms that verapamil inhibits cell growth and atherosclerotic plaque formation reverses cardiac hypertrophy.