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Objective To establish rat C6 brain-tumor models and f ind a simple reliable index to judge tumor volume. Methods C6 cell suspension (10?μl) containing 10?g/L agarose and 1×10 6 cells was i njected into the right caudate nucleus of the rat brain by a stereotaxic method . After implantation, rats were observed and given MRI scans. Rats were perfus ed with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and b efore natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hemato xylin and eosin (HE) stains. Results Fifty implanted r ats had 100% yield of intracerebral growth, with distant metast asis of 0% to 4%. Conclusion A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size
Objective To establish rat C6 brain-tumor models and f ind a simple reliable index to judge tumor volume. Methods C6 cell suspension (10 μl) containing 10 μg / L agarose and 1 × 10 6 cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfus ed with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and b efore natural death. All brains, lungs Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains. Results Fifty implanted r ats had 100% yield of intracerebral growth, with distant metastasis asis of 0% to 4% . Conclusion A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size