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目的:探讨在高浓度胰岛素条件下,体外培养的兔视网膜Müller细胞的血管内皮细胞生长因子(vas-cularendothelialgrowthfactor,VEGF)的变化。方法:在不同浓度胰岛素条件下(4,8,12kU/L),体外培养兔视网膜Müller细胞,采用免疫细胞化学法、原位杂交法,定性测定Müller细胞分泌VEGF的变化,采用ELISA方法,定量测定Müller细胞分泌VEGF的变化。结果:高浓度胰岛素能明显增强VEGF的表达(P< 0.05)。结论:高浓度胰岛素可能通过刺激Müller细胞编码VEGF基因的转录,进而增强VEGF蛋白的表达,而在糖尿病视网膜病变的新生血管生成中发挥重要作用。
Objective: To investigate the changes of vas-cular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells under high concentration of insulin. Methods: Rabbit retinal Müller cells were cultured in vitro with different concentrations of insulin (4, 8, 12 kU / L). The changes of VEGF secreted by Müller cells were detected by immunocytochemistry and in situ hybridization. The changes of VEGF secreted by Müller cells were measured. Results: High concentration of insulin significantly enhanced the expression of VEGF (P <0.05). CONCLUSION: High concentration of insulin may play an important role in neovascularization of diabetic retinopathy possibly by stimulating the transcription of VEGF gene by Müller cells, thereby enhancing the expression of VEGF protein.