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对日本商品膜荚黄芪 Astragalus mere-branaceus Bunge 根依次用甲醇和乙醚提取,乙醚提取液经硅胶柱层析,以苯-乙酸乙酯洗脱,得到4种异黄酮类化合物,即阿佛洛莫生(afrormosin)、calycosin、芒柄花黄素(for-mononetin)和芳香膜菊素(odoratin)。对这4种化合物的抑制脂质过氧化作用进行研究的结果表明,在对血红蛋白与过氧化氢反应诱导的卵磷脂过氧化抑制实验中,calycosin 在浓度为250μmol/L 时抑制率达(81.1±
The Astragalus mere-branaceus Bunge root was extracted from the Astragalus mere-branaceus Bunge root of the Japanese commercial film, followed by extraction with methanol and ether. The ether extract was subjected to silica gel column chromatography and eluted with benzene-ethyl acetate to obtain 4 kinds of isoflavone compounds, namely, Aphromycin. Afrormosin, calycosin, for-mononetin, and odoratin. The results of studies on lipid peroxidation inhibition of these four compounds showed that the inhibition rate of calycosin was (81.1±) at a concentration of 250 μmol/L in a lecithin peroxidation inhibition experiment induced by a reaction between hemoglobin and hydrogen peroxide.