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应用组织化学反应显示繁殖期中华鳖输卵管碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、碳酸酐酶(CA)、过氧化氢酶(CAT)以及三磷酸腺苷酶(ATPase)的分布特点,在组织和细胞水平上分析这些酶的分布意义。结果显示,5种酶在繁殖期的输卵管中均有分布,但在各段组织中的反应部位及反应强弱不同。ALP主要分布于血管壁和腺体胞质,在排卵期的子宫部和有精子储存的部位,阳性反应较强;ACP主要分布于输卵管上皮和腺体;CA在各段上皮的纤毛和固有膜的腺体均有分布,但在精子储存部位反应较弱;CAT在各段腺体呈阳性反应,尤其储精小管的腺体反应较强;ATPase在各段上皮纤毛及固有膜腺体阳性反应较强。表明,这些酶在卵子的形成、运输以及精子储存等分泌具有一定生物学作用。
The histochemical analysis of the distribution of alkaline phosphatase (ALP), acid phosphatase (ACP), carbonic anhydrase (CA), catalase (CAT) The distribution of these enzymes is analyzed at the tissue and cellular level. The results showed that the five kinds of enzymes in the reproductive phase of the fallopian tubes are distributed, but in each segment of the reaction site and the reaction strength is different. ALP is mainly distributed in the blood vessel wall and gland cytoplasm, positive in ovulation uterus and sperm storage site, ACP is mainly distributed in fallopian tube epithelium and gland; CA in each segment epithelial cilia and lamina propria Of the glands are distributed, but the reaction in the sperm storage site is weak; CAT in each segment of the gland positive reaction, in particular, the seminiferous tubules of the gland response is stronger; ATPase in each segment epithelial cilia and lamina propria gland positive reaction Strong. Show that these enzymes in the egg formation, transport and secretion of secreted sperm have some biological effects.