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目的研究ｐ１６基因产物在人肺癌中的表达及其意义。方法用兔抗人ｐ１６多克隆抗体，以ＳＰ免疫组织化学方法检测肺癌组织中ｐ１６蛋白表达。结果ｐ１６在肺癌中表达阳性率为５８．５％（６２／１０６），在腺癌中的表达较鳞癌和小细胞癌高（Ｐ＜０．０５）。在鳞癌和腺癌中ｐ１６表达阳性率随着分化程度的降低而下降，高分化和低分化鳞癌之间ｐ１６阳性率有非常显著的差异（Ｐ＜０．０１）；鳞癌和腺癌中ｐ１６阳性率与有无淋巴结转移有密切关系（Ｐ＜０．０５）；与肺癌病理分期无明显关系。结论ｐ１６基因在不同类型肺癌的发生过程中作用不同，且与肺癌分化不良和有无淋巴结转移有密切关系。
Objective To study the expression and significance of p16 gene product in human lung cancer. Methods Rabbit anti-human p16 polyclonal antibody was used to detect the expression of p16 protein in lung cancer tissues by SP immunohistochemistry. Results The positive expression rate of p16 in lung cancer was 58.5% (62/106). The expression of p16 in adenocarcinoma was higher than that in squamous cell carcinoma and small cell carcinoma (P<0.05). The positive rate of p16 expression in squamous cell carcinoma and adenocarcinoma decreased with the degree of differentiation. The positive rate of p16 between highly differentiated and poorly differentiated squamous cell carcinomas was significantly different (P<0.01); squamous cell carcinoma and adenocarcinoma The positive rate of p16 was closely related to the presence or absence of lymph node metastasis (P<0.05); it was not significantly related to the pathological stage of lung cancer. Conclusion The p16 gene plays a different role in the development of different types of lung cancer, and it is closely related to poor lung cancer differentiation and lymph node metastasis.