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目的:探讨G蛋白偶联受体56(GPR56)表达与人结肠癌临床病理资料的关系。方法:75例人结肠癌及癌旁组织的组织芯片,应用免疫组化SP法检测GPR56蛋白表达情况;应用Western-blot检测20例人结肠癌组织及癌旁组织中GPR56的表达。结果:免疫组化显示结肠癌组织中GPR56蛋白表达明显高于其在癌旁组织中的表达(MOD值0.052±0.030比0.045±0.026,t=4.529,P<0.05);结合临床病理参数进行相关分析,结果75例不同分化程度的结肠癌组织中GPR56蛋白表达上调与肿瘤的分化程度、淋巴结转移有无及远处转移有无、浸润程度、pTNM分期有关(P<0.05);而与年龄、性别无关(P>0.05),GPR56蛋白表达与结肠癌患者生存期也无明显关系(t=0.841,P>0.05)。Western-blot分析结果显示GPR56蛋白在结肠癌组织(0.927±0.063)中的表达水平高于癌旁组织(0.797±0.050,P<0.05)。结论:GPR56表达升高与结肠癌发生、发展有关,可能是结肠癌恶性生物学行为的指标。
Objective: To investigate the relationship between the expression of G protein-coupled receptor 56 (GPR56) and the clinicopathological data of human colon cancer. Methods: Tissue microarrays were obtained from 75 human colon cancer tissues and adjacent tissues. The expression of GPR56 protein was detected by immunohistochemical SP method. The expression of GPR56 in 20 human colon carcinomas and adjacent normal tissues was detected by Western-blot. Results: Immunohistochemistry showed that the expression of GPR56 protein in colon cancer tissues was significantly higher than that in the adjacent non-cancerous tissues (MOD value 0.052 ± 0.030 vs 0.045 ± 0.026, t = 4.529, P <0.05), and correlated with clinicopathological parameters Results The upregulation of GPR56 protein in 75 cases of different degree of differentiation of colon cancer was related to the differentiation of tumor, lymph node metastasis with or without distant metastasis, infiltration degree and pTNM stage (P <0.05) There was no significant correlation between GPR56 protein expression and survival of patients with colon cancer (t = 0.841, P> 0.05). The results of Western-blot showed that the expression of GPR56 protein in colon cancer tissues (0.927 ± 0.063) was higher than that in adjacent tissues (0.797 ± 0.050, P <0.05). Conclusion: The increased expression of GPR56 is associated with the occurrence and development of colon cancer, which may be an indicator of the malignant biological behavior of colon cancer.