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目的测定川芎挥发油中藁本内酯在大鼠体内的药动学。方法采用RP-HPLC法,以蛇床子素为内标物测定大鼠口服川芎挥发油的β-环糊精包合物后藁本内酯的血药浓度,使用3P97药动学软件计算其药动学参数。结果藁本内酯在大鼠体内符合二室模型,主要的药动学参数:tl/2(α)为(1.429±1.161)h,tl/2(β)为(6.877±2.275)h,t(peak)为(3.401±1.951)h,AUC为(70.87±25.92)μg/mL.h。结论以蛇床子素为内标,RP-HPLC法能够准确、灵敏地测定藁本内酯在大鼠体内的药动学。
Objective To determine the pharmacokinetics of ligustilide in the volatile oil of Ligusticum chuanxiong Hort in rats. Methods The plasma concentration of ligustilide β-cyclodextrin inclusion complex was determined by RP-HPLC with osthole as the internal standard. The pharmacokinetics were calculated by 3P97 pharmacokinetic software Learning parameters. Results The ligustilide was in accordance with the two-compartment model in rats. The main pharmacokinetic parameters were as follows: t1 / 2 (α) = 1.429 ± 1.161 h, t1 / 2 (β) = (6.877 ± 2.275) (3.401 ± 1.951) h and AUC of (70.87 ± 25.92) μg / mL.h. Conclusion Osthol can be used as an internal standard to determine the pharmacokinetics of ligustilide in rats by RP-HPLC.