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目的探讨血管内皮生长因子-D(Vessel endothelium growth factor D,VEGF-D)在喉癌组织中的表达规律及与淋巴管密度(LVD)和淋巴转移之间的关系及其在喉癌预后中的意义。方法以45例经病理确诊的喉癌组织为实验组,以20例喉良性病变组织为对照组,采用免疫组化SP法对上述组织中VEGF-D蛋白的表达进行分析,5’-核苷酸酶-碱性磷酸酶双重染色法(5’-Nase-ALP)计数淋巴管密度(LVD),并结合临床病理特征和生存资料进行相关分析。结果在喉癌组织中VEGF-D的阳性率为42.2%,明显高于在良性病变组织的表达水平(<0.05)。VEGF-D表达与瘤内和瘤周LVD、淋巴结转移、淋巴管浸润显著相关(<0.01),与年龄、性别、肿瘤部位及组织学分级无关(>0.05)。Kaplan-Meier生存曲线分析表明VEGF-D的表达与生存率呈负相关(<0.05)。结论 VEGF-D促进喉癌淋巴管生成和淋巴管转移,可以成为喉癌评估预后的重要分子指标。
Objective To investigate the expression of vascular endothelial growth factor-D (VEGF-D) in laryngeal carcinoma and its relationship with lymphatic vessel density (LVD) and lymphatic metastasis and its relationship with the prognosis of laryngeal carcinoma significance. Methods Totally 45 cases of laryngeal carcinoma confirmed by pathology were selected as experimental group and 20 cases of laryngeal benign lesion as control group. The expression of VEGF-D protein was analyzed by immunohistochemical SP method. The lymphatic vessel density (LVD) was counted by enzyme-alkaline phosphatase double staining (5-Nase-ALP), and the correlation analysis was made based on the clinicopathological features and survival data. Results The positive rate of VEGF-D in laryngeal carcinoma was 42.2%, which was significantly higher than that in benign lesions (<0.05). The expression of VEGF-D was significantly correlated with LVD, lymph node metastasis and lymphatic invasion (P <0.01), but not with age, sex, tumor location and histological grade (> 0.05). Kaplan-Meier survival curve analysis showed that the expression of VEGF-D and the survival rate was negatively correlated (<0.05). Conclusion VEGF-D can promote the lymphangiogenesis and lymphatic metastasis of laryngeal carcinoma, which may be an important molecular index for evaluating the prognosis of laryngeal cancer.