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以沙冬青种子为试材,采用恒温培养法研究了不同浓度NaCl(10、20、30、40、50、60、70、80、90、100mmol/L)对沙冬青种子萌发及幼苗生长的影响,并观察了萌发恢复率。结果表明:在NaCl模拟盐胁迫下,沙冬青种子萌发率与NaCl浓度呈极显著负相关(R2=0.9602),NaCl浓度低于30mmol/L时,对沙冬青种子的萌发影响较小,其中10mmol/L NaCl能提高沙冬青种子萌发率,而高于80mmol/L浓度的NaCl对种子萌发、幼苗鲜重和胚根生长均产生抑制作用。沙冬青幼苗根长、地上部分鲜重、根鲜重以及根冠比随NaCl浓度的增加呈下降趋势;可溶性糖含量、脯氨酸含量、保护性酶(POD、SOD)活性随NaCl浓度的增加表现为先增加后降低趋势;种子浸提液丙二醛(MDA)含量和CAT活性随NaCl浓度的增加逐渐上升;幼苗根系活力(TTC)与NaCl溶液浓度呈单峰曲线,在30mmol/L出现峰值。解除胁迫条件,不同NaCl处理沙冬青种子复水萌发率平均达15.8%,复200mg/L赤霉素萌发率平均达23.1%。综合来看,沙冬青种子恢复萌发率与NaCl浓度之间呈极显著负相关,表明一定浓度盐胁迫下沙冬青种子仍具有较高的萌发潜力和抗盐特性,但过高浓度的NaCl导致沙冬青种子失活。
The effects of different concentrations of NaCl (10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 mmol / L) on the seed germination and seedling growth of A. astigma , And observed the germination recovery rate. The results showed that there was a significant negative correlation between the seed germination rate and the NaCl concentration (R2 = 0.9602) under NaCl simulated salinity stress. When the NaCl concentration was below 30 mmol / L, the seed germination was less affected, and 10 mmol / L NaCl could improve the seed germination rate of A. asiatica, while the NaCl concentration above 80 mmol / L could inhibit seed germination, seedling fresh weight and radicle growth. The root length, fresh weight, root fresh weight and root / shoot ratio of A. astigii seedlings decreased with the increase of NaCl concentration. The activities of soluble sugar, proline, protective enzyme (POD, SOD) increased with the increase of NaCl concentration The content of malondialdehyde (MDA) and the activity of CAT increased gradually with the increase of NaCl concentration. The root activity (TTC) and NaCl concentration of seedling showed a single peak curve, and appeared at 30mmol / L Peak. With the release of stress, the germination rate of seedlings treated with different NaCl treatments reached 15.8% on average, and the average germination rate of gibberellin at 200mg / L reached 23.1%. Taken together, there was a significant negative correlation between seed germination rate and NaCl concentration in A. sojae, indicating that Salvia miltiorrhiza seed had higher germination potential and salt resistance under salt stress. However, Inactivation of seeds.