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目的研究回流提取法中各因素对八角莲中鬼臼毒素,4’-去甲鬼臼毒素提取效果的影响。方法采用L9(34)正交试验,以鬼臼毒素,4’-去甲鬼臼毒素含量为指标,确定回流提取法各因素对八角莲中有效物质提取效果的影响,结果八角莲最佳回流提取工艺为:料液比为1∶5,温度80℃,90%乙醇提取3次每次1h。
Objective To study the effect of various factors on the extraction of podophyllotoxin and 4’-demethyl podophyllotoxin in Octagonal lotus by reflux extraction. Methods Using L9 (34) orthogonal test and podophyllotoxin and 4’-demethiodia podophyllotoxin as indexes, the effects of various factors of reflux extraction on the extraction of effective substances from the body of Octagonal lotus were determined. The extraction process was as follows: the ratio of solid to liquid was 1: 5, the temperature was 80 ℃, and the extraction of 90% ethanol was performed 3 times for 1 hour each time.